Medium data

Search for medium no. = [822].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

822   FERMENTATIVE CELLULOLYTIC ANAEROBES MEDIUM

KCl  0.33    g
NH4Cl  0.25    g
MgSO4·7H2O  0.6     g
NaCl  0.4     g
CaCl2·2H2O  0.1     g
Trace minerals (see Medium No. 151)  10.0     ml
Yeast extract  0.2     g
Resazurin  0.25    mg
1.0 M Phosphate buffer (see below)  1.0     ml
MOPS  2.09    g
Distilled water  910.0     ml
Mix components and bring to a boil for 5-10 sec, then, cool down under a N2-CO2 (80:20, v/v) gas stream. Dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
10% Cellobiose solution*  40.0     ml
Trace vitamins (see Medium No. 197)*  10.0     ml
5% NaHCO3 solution*  10.0     ml
5% Na2S·9H2O solution  10.0     ml
5% L-Cysteine·HCl·H2O  10.0     ml
1.0 M Phosphate buffer:
Mix 50 ml of 1.0 M KH2PO4 and 50 ml of 1.o M Na2HPO4 to prepare 100 ml of 1.0 M phosphate buffer.



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