Medium data

Search for medium no. = [808].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

808 SULFURIMONAS GD1 MEDIUM

NaCl	`5.5`	g
MgCl₂·6H₂O	`2.28`	g
CaCl₂·2H₂O	`0.34`	g
KCl	`0.15`	g
Na₂SO₄	`0.91`	g
NaHCO₃	`0.20`	g
KBr	`0.023`	g
H₃BO₃	`5.7`	mg
SrCl₂·6H₂O	`9.1`	mg
NH₄Cl	`4.9`	mg
KH₂PO₄	`1.2`	mg
NaF	`0.67`	mg
HEPES	`2.38`	g
Distilled water	`1.0`	L

Mix ingredients and adjust pH to 7.3. Fill the medium in a glass bottle, bubble with N₂ for 30 min, then, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):

FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
10% KNO₃ solution	`10.0`	ml
10% Na₂S₂O₃·5H₂O solution	`10.0`	ml