Medium data

Search for medium no. = [784].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

784 DESULFOSPOROSINUS ACIDOPHILUS MEDIUM

(NH₄)₂SO₄	`0.45`	g
KH₂PO₄	`0.05`	g
MgSO₄·7H₂O	`0.5`	g
KCl	`0.05`	g
Yeast extract	`0.5`	g
Ca(NO₃)₂·4H₂O	`0.014`	g
Na₂SO₄	`3.0`	g
Trace element solution (see Medium No. 439)	`1.0`	ml
L-Cysteine·HCl·H₂O	`0.5`	g
Resazurin	`1.0`	mg
Distilled water	`900.0`	ml

Mix components thoroughly, distribute the medium into culture vessels (e.g., 4.5 ml of the medium in the Hungate tubes) under a N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 900 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized):

5% Na₂HPO₄ solution	`20.0`	ml
25% KH₂PO₄ solution	`40.0`	ml
1 M Fructose solution	`20.0`	ml
Trace vitamins (see Medium No. 197)*	`20.0`	ml
5% Na₂S·9H₂O solution	`8.0`	ml

Check final pH of the medium to be around 5.8.