Medium data

Search for medium no. = [439].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  1.0     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.1     g
NH4Cl  0.25    g
KH2PO4  2.0     g
KCl  0.5     g
Yeast extract  0.5     g
Na2SO4  4.0     g
Trace element solution (see below)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly and adjust pH to 7.0. Bring to a boil, and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
1.0 M Sodium lactate solution  20.0     ml
Vitamin solution* (see Medium No. 403)  1.0     ml
Thiamine solution* (see Medium No. 403)  1.0     ml
Vitamin B12 solution* (see Medium No. 403)  1.0     ml
8.0% NaHCO3 solution*  20.0     ml
Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  2.0     ml
Trace element solution:
HCl (25%)  12.5     ml
FeSO4·7H2O  2.1     g
H3BO3  30.0     mg
MnCl2·4H2O  100.0     mg
CoCl2·6H2O  190.0     mg
NiCl2·6H2O  24.0     mg
CuCl2·2H2O  2.0     mg
ZnSO4·7H2O  144.0     mg
Na2MoO4·2H2O  36.0     mg
Distilled water  987.0     ml

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