Medium data
Search for medium no. = [439].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
439 DESULFOVIBRIO AEROTOLERANS DVO5 MEDIUM
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.4 | g |
| CaCl2·2H2O | 0.1 | g |
| NH4Cl | 0.25 | g |
| KH2PO4 | 2.0 | g |
| KCl | 0.5 | g |
| Yeast extract | 0.5 | g |
| Na2SO4 | 4.0 | g |
| Trace element solution (see below) | 1.0 | ml |
| Selenite-tungstate solution (see Medium No. 431) | 1.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 7.0. Bring to a boil, and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 10 ml medium in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, anaerobically and aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
| 1.0 M Sodium lactate solution | 20.0 | ml |
| Vitamin solution* (see Medium No. 403) | 1.0 | ml |
| Thiamine solution* (see Medium No. 403) | 1.0 | ml |
| Vitamin B12 solution* (see Medium No. 403) | 1.0 | ml |
| 8.0% NaHCO3 solution* | 20.0 | ml |
Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
| 5% Na2S·9H2O solution | 2.0 | ml |
Trace element solution:
| HCl (25%) | 12.5 | ml |
| FeSO4·7H2O | 2.1 | g |
| H3BO3 | 30.0 | mg |
| MnCl2·4H2O | 100.0 | mg |
| CoCl2·6H2O | 190.0 | mg |
| NiCl2·6H2O | 24.0 | mg |
| CuCl2·2H2O | 2.0 | mg |
| ZnSO4·7H2O | 144.0 | mg |
| Na2MoO4·2H2O | 36.0 | mg |
| Distilled water | 987.0 | ml |
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