Medium data
Search for medium no. = [780].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
780 DESULFOTOMACULUM VARUM MEDIUM
| NH4Cl | 1.0 | g |
| K2HPO4 | 0.6 | g |
| KH2PO4 | 0.3 | g |
| MgCl2·6H2O | 0.1 | g |
| CaCl2·2H2O | 0.1 | g |
| NaCl | 1.0 | g |
| HEPES | 12.0 | g |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| Trace mineral solution (see Medium No. 684) | 1.0 | ml |
| Yeast extract | 0.2 | g |
| Na2SO4 | 2.84 | g |
| Distilled water | 1.0 | L |
Mix components thoroughly, and adjust pH to 7.0. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solution (autoclaved under a N2 atmosphere):
| 20% (w/v) Glycerin solution | 10.0 | ml |
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