Medium data

Search for medium no. = [780].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

780   DESULFOTOMACULUM VARUM MEDIUM

NH4Cl  1.0      g
K2HPO4  0.6      g
KH2PO4  0.3      g
MgCl2·6H2O  0.1      g
CaCl2·2H2O  0.1      g
NaCl  1.0      g
HEPES  12.0      g
Trace vitamins (see Medium No. 197)  1.0      ml
Trace mineral solution (see Medium No. 684)  1.0      ml
Yeast extract  0.2      g
Na2SO4  2.84     g
Distilled water  1.0      L
Mix components thoroughly, and adjust pH to 7.0. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solution (autoclaved under a N2 atmosphere):
20% (w/v) Glycerin solution  10.0      ml



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