Medium data
Search for medium no. = [684].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
684 ANAEROBIC TYEG MEDIUM
| KH2PO4 | 0.75 | g |
| K2HPO4 | 1.5 | g |
| NaCl | 0.9 | g |
| MgCl2·6H2O | 0.2 | g |
| NH4Cl | 0.9 | g |
| Trace mineral solution (see below) | 9.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| 10% FeSO4·7H2O solution | 5.0 | µl |
| Tryptone (BD-Difco) | 2.0 | g |
| Yeast extract | 2.0 | g |
| Glucose | 2.0 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 7.5. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter):
| 5% Na2S·9H2O solution | 8.0 | ml |
Trace mineral solution:
| Nitrilotriacetic acid | 12.5 | g |
| FeCl2·4H2O | 0.2 | g |
| MnCl2·4H2O | 0.1 | g |
| CoCl2·6H2O | 0.02 | g |
| CaCl2·2H2O | 0.1 | g |
| ZnCl2 | 0.1 | g |
| CuCl2 | 0.2 | g |
| H3BO3 | 0.01 | g |
| Na2MoO4·2H2O | 0.01 | g |
| NaCl | 1.0 | g |
| Na2SeO3·5H2O | 0.02 | g |
| Distilled water | 1.0 | L |
Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals.
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