Medium data

Search for medium no. = [684].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

684 ANAEROBIC TYEG MEDIUM

KH₂PO₄	`0.75`	g
K₂HPO₄	`1.5`	g
NaCl	`0.9`	g
MgCl₂·6H₂O	`0.2`	g
NH₄Cl	`0.9`	g
Trace mineral solution (see below)	`9.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
10% FeSO₄·7H₂O solution	`5.0`	µl
Tryptone (BD-Difco)	`2.0`	g
Yeast extract	`2.0`	g
Glucose	`2.0`	g
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Mix components and adjust pH to 7.5. Bring to a boil, then cool down and distribute into culture vessels under a N₂ gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N₂ atmosphere) into the medium (per liter):

5% Na₂S·9H₂O solution 8.0 ml

Trace mineral solution:

Nitrilotriacetic acid	`12.5`	g
FeCl₂·4H₂O	`0.2`	g
MnCl₂·4H₂O	`0.1`	g
CoCl₂·6H₂O	`0.02`	g
CaCl₂·2H₂O	`0.1`	g
ZnCl₂	`0.1`	g
CuCl₂	`0.2`	g
H₃BO₃	`0.01`	g
Na₂MoO₄·2H₂O	`0.01`	g
NaCl	`1.0`	g
Na₂SeO₃·5H₂O	`0.02`	g
Distilled water	`1.0`	L

Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals.