Medium data

Search for medium no. = [684].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

684   ANAEROBIC TYEG MEDIUM

KH2PO4  0.75    g
K2HPO4  1.5     g
NaCl  0.9     g
MgCl2·6H2O  0.2     g
NH4Cl  0.9     g
Trace mineral solution (see below)  9.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
10% FeSO4·7H2O solution  5.0     µl
Tryptone (BD-Difco)  2.0     g
Yeast extract  2.0     g
Glucose  2.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components and adjust pH to 7.5. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter):
5% Na2S·9H2O solution  8.0     ml
Trace mineral solution:
Nitrilotriacetic acid  12.5     g
FeCl2·4H2O  0.2     g
MnCl2·4H2O  0.1     g
CoCl2·6H2O  0.02    g
CaCl2·2H2O  0.1     g
ZnCl2  0.1     g
CuCl2  0.2     g
H3BO3  0.01    g
Na2MoO4·2H2O  0.01    g
NaCl  1.0     g
Na2SeO3·5H2O  0.02    g
Distilled water  1.0     L
Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals.