Medium data

Search for medium no. = [755].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

755   MMJ-S304 MEDIUM

Modified MJ synthetic seawater (see Medium No. 492)  1.0     L
Yeast extract  1.0     g
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components and adjust pH to 6.5 with NaOH. Bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following solutions (autoclaved or *filter-sterilize) from anaerobic stocks:
8% NaHCO3 solution*  12.5     ml
5% Na2S·9H2O solution  10.0     ml
Adjust pH of the medium to 6.0 - 6.5, if necessary. Pressurize the inoculated bottles to 200 kPa N2-CO2 (4:1, v/v).

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