Medium data

Search for medium no. = [492].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

492   MMJ-TL MEDIUM

Modified MJ synthetic seawater (see below)  1.0     L
Sulfur (powder)  3.0     g
Resazurin  1.0     mg
Mix ingredients except sulfur, adjust pH to 7.0 with NaOH, and autoclave under a N2 atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
Trace vitamins* (see Medium No. 197)  10.0     ml
1.0 M Sodium acetate solution  10.0     ml
1% Yeast extract solution  10.0     ml
Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g. 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (autoclaved and stocked under a N2 atmosphere):
5% Na2S·9H2O solution  10.0     ml
Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Modified MJ synthetic seawater:
NaCl  30.0     g
K2HPO4  0.14    g
CaCl2·2H2O  0.5     g
NH4Cl  0.5     g
MgSO4·7H2O  3.4     g
MgCl2·6H2O  4.18    g
KCl  0.33    g
FeSO4·7H2O  0.01    g
NiCl2·6H2O  1.0     mg
Na2SeO3·5H2O  1.0     mg
Trace minerals (see Medium No. 151)  10.0     ml
Distilled water  1.0     L



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