Medium data

Search for medium no. = [753].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

753 DEHALOSPIRILLUM MEDIUM

Solution A:

Na₂SO₄	`0.7`	g
K₂HPO₄	`0.2`	g
NH₄Cl	`0.25`	g
NaCl	`0.25`	g
MgCl₂·6H₂O	`0.4`	g
KCl	`0.5`	g
CaCl₂·2H₂O	`0.15`	g
Resazurin	`0.5`	mg
Yeast extract	`2.0`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
L-Cysteine·HCl·H₂O	`0.05`	g
FeSO₄·7H₂O	`0.025`	g
Distilled water	`900.0`	ml

Mix components except L-cysteine·HCl·H₂O and FeSO₄·7H₂O. Bring to a boil for a few seconds and cool down under a N₂ atmosphere. While gassing with N₂, add L-cysteine·HCl·H₂O and FeSO₄·7H₂O to the solution, distribute into culture vessels (e.g., 18 ml in 50 ml serum bottles) under a N₂ atmosphere, then, seal butyl rubber stoppers and autoclave. Aseptically and anaerobically add per liter (final) the following sterile solutions (autoclaved or *filter-sterilized) from anaerobic stocks:

0.1 M Potassium phosphate buffer, pH 7.5	`10.0`	ml
Trace vitamins (see Medium No. 197)*	`10.0`	ml
Vitamin B₁₂ solution (see Medium No. 403)*	`1.0`	ml
8% NaHCO₃ solution*	`42.0`	ml
25% Sodium pyruvate solution*	`18.0`	ml
16% Sodium fumarate solution*	`40.0`	ml

Adjust pH of the medium to 7.3 - 7.7, if necessary.