Medium data

Search for medium no. = [751].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

751 MODIFIED NAUTILIA LITHOTROPHICA MEDIUM

NaCl	`25.0`	g
NaNO₃	`1.0`	g
NH₄Cl	`0.33`	g
KCl	`0.33`	g
CaCl₂·2H₂O	`0.33`	g
MgCl₂·6H₂O	`0.33`	g
HEPES	`2.38`	g
Resazurin	`1.0`	mg
Trace minerals (see Medium No. 151)	`10.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
Sulfur (powder)	`12.0`	g
Distilled water	`900.0`	ml

Mix components except sulfur, adjust pH to 6.8 and autoclave under a N₂-CO₂ (4:1, v/v) atmosphere. Distribute medium into culture vessels (e.g., 18 ml in 100 ml serum bottles) containing appropriate amount of sulfur under a N₂-CO₂ (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) to the above described basal medium (900 ml):

15% Sodium formate solution	`20.0`	ml
5% NaHCO₃ solution*	`50.0`	ml
Trace vitamins (see Medium No. 197)*	`10.0`	ml
3% Na₂S·9H₂O solution	`20.0`	ml

Readjust pH of the medium to 6.8, if necessary.