Medium data

Search for medium no. = [718].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

718   SPOROLITUUS THERMOPHILUS MEDIUM

KH2PO4  0.75    g
K2HPO4  1.5     g
NaCl  0.9     g
MgCl2·6H2O  0.2     g
NH4Cl  0.9     g
Trace mineral solution (see Medium No. 684)  9.0     ml
Trace vitamins (see Medium No. 197)  5.0     ml
10% FeSO4·7H2O solution  5.0     µl
Tryptone (BD-Difco)  2.0     g
Yeast extract  2.0     g
Sodium citrate  2.0     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly. Bring to a boil, cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter):
5% Na2S·9H2O solution  4.0     ml



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