Medium data

Search for medium no. = [702].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

702   METHANOBACTERIUM MEDIUM (II)

NH4Cl  1.0     g
K2HPO4  0.3     g
KH2PO4  0.3     g
MgCl2·6H2O  0.2     g
CaCl2·2H2O  0.1     g
KCl  0.1     g
NaCl  0.6     g
Sodium acetate  0.5     g
Yeast extract  0.5     g
Resazurin  0.5     mg
Trace minerals (see Medium No. 151)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
L-Cysteine·HCl·H2O  0.5     g
Distilled water  1.0     L
Mix components, bring to a boil for several seconds and cool down under a stream of H2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
3% Na2S·9H2O solution  10.0     ml
Check pH of the medium to be about 7.0. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).