Medium data
Search for medium no. = [702].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
702 METHANOBACTERIUM MEDIUM (II)
| NH4Cl | 1.0 | g |
| K2HPO4 | 0.3 | g |
| KH2PO4 | 0.3 | g |
| MgCl2·6H2O | 0.2 | g |
| CaCl2·2H2O | 0.1 | g |
| KCl | 0.1 | g |
| NaCl | 0.6 | g |
| Sodium acetate | 0.5 | g |
| Yeast extract | 0.5 | g |
| Resazurin | 0.5 | mg |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Distilled water | 1.0 | L |
Mix components, bring to a boil for several seconds and cool down under a stream of H2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 25.0 | ml |
| 3% Na2S·9H2O solution | 10.0 | ml |
Check pH of the medium to be about 7.0. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
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