Medium data

Search for medium no. = [696].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

696   THERMODESULFATATOR ATLANTICUS MEDIUM

NaCl  20.0     g
MgCl2·6H2O  3.0     g
Na2SO4  3.0     g
KCl  0.5     g
NH4Cl  0.3     g
KH2PO4  0.1     g
PIPES  1.73    g
CaCl2·2H2O  0.11    g
Trace minerals (see Medium No. 151)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Na2S·9H2O  0.3     g
Distilled water  1.0     L
Mix components except Na2S·9H2O and adjust pH to 6.7. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a gas stream of H2-CO2 (4:1, v/v), seal with butyl rubber stoppers and autoclave. Separately autoclave 3% Na2S·9H2O solution under a N2 gas atmosphere. Prior to inoculation, reduce the medium by the addition of the Na2S·9H2O solution. Pressurized the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).