Medium data
Search for medium no. = [696].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
696 THERMODESULFATATOR ATLANTICUS MEDIUM
| NaCl | 20.0 | g |
| MgCl2·6H2O | 3.0 | g |
| Na2SO4 | 3.0 | g |
| KCl | 0.5 | g |
| NH4Cl | 0.3 | g |
| KH2PO4 | 0.1 | g |
| PIPES | 1.73 | g |
| CaCl2·2H2O | 0.11 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Na2S·9H2O | 0.3 | g |
| Distilled water | 1.0 | L |
Mix components except Na2S·9H2O and adjust pH to 6.7. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a gas stream of H2-CO2 (4:1, v/v), seal with butyl rubber stoppers and autoclave. Separately autoclave 3% Na2S·9H2O solution under a N2 gas atmosphere. Prior to inoculation, reduce the medium by the addition of the Na2S·9H2O solution. Pressurized the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).
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