Medium data

Search for medium no. = [687].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

687   MB MEDIUM WITH METHANOL

Solution A:
NaCl  10.0     g
Yeast extract  2.0     g
Trypticase peptone  2.0     g
NH4Cl  1.0     g
MgCl2·6H2O  1.0     g
KCl  0.5     g
CaCl2·2H2O  0.4     g
K2HPO4  0.4     g
Resazurin  0.5     mg
Trace minerals (see Medium No. 151)  10.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
NaHCO3  4.0     g
Distilled water  920.0     ml
Mix components except NaHCO3, bring to a boil for several seconds and cool down under a stream of N2-CO2 (80:20). Add NaHCO3, mix thoroughly and distribute the medium into culture vessels under the same gas mixture. Seal with butyl rubber stoppers and autoclave. Finally, add per liter the following sterile solutions (autoclaved or *filter-sterilized):
Methanol solution (see below)*  50.0     ml
Trace vitamins (see Medium No. 197)*  10.0     ml
5% L-Cysteine·HCl·H2O  5.0     ml
5% Na2S·9H2O solution  5.0     ml
Methanol solution:
Methanol  2.28    ml
Distilled water  50.0     ml