Medium data

Search for medium no. = [673].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

673   METHANOPYRUS MEDIUM FOR STRAIN 116

NaCl  20.0     g
K2HPO4  0.07    g
KH2PO4  0.09    g
CaCl2·2H2O  0.8     g
NH4Cl  0.3     g
MgSO4·7H2O  6.0     g
MgCl2·6H2O  1.0     g
KCl  0.33    g
NiCl2·6H2O  5.0     mg
Na2SeO3·5H2O  5.0     mg
Fe2(SO4)3·xH2O  0.05    g
FeSO4·7H2O  0.01    g
Na2WO4·2H2O  5.0     mg
Resazurin  1.0     mg
Marine trace elements (see Medium No. 216)  30.0     ml
Iron (powder, 20 - 60 mesh, Nacalai)  20.0     g
NaHCO3  2.0     g
Trace vitamins (see Medium No. 197)  10.0     ml
Trace mineral solution (see Medium No. 368)  10.0     ml
Na2S·9H2O  0.5     g
Distilled water  970.0     ml
Mix components except iron powder and filter-sterilize. Dispense the medium and iron powder (autoclaved) into sterile culture vessels (typically, 15 - 20 ml medium in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.01 volume of 5% Na2S·9H2O solution (neutralized with H2SO4, autoclaved and stored under a N2 atmosphere). Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20, v/v).