Medium data

Search for medium no. = [673].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

673 METHANOPYRUS MEDIUM FOR STRAIN 116

NaCl	`20.0`	g
K₂HPO₄	`0.07`	g
KH₂PO₄	`0.09`	g
CaCl₂·2H₂O	`0.8`	g
NH₄Cl	`0.3`	g
MgSO₄·7H₂O	`6.0`	g
MgCl₂·6H₂O	`1.0`	g
KCl	`0.33`	g
NiCl₂·6H₂O	`5.0`	mg
Na₂SeO₃·5H₂O	`1.0`	mg
Fe₂(SO₄)₃·xH₂O	`0.05`	g
FeSO₄·7H₂O	`0.01`	g
Na₂WO₄·2H₂O	`5.0`	mg
Resazurin	`1.0`	mg
Marine trace elements (see Medium No. 216)	`30.0`	ml
Iron (powder, 20 - 60 mesh, Nacalai)	`20.0`	g
NaHCO₃	`2.0`	g
Trace vitamins (see Medium No. 197)	`10.0`	ml
Trace mineral solution (see Medium No. 368)	`10.0`	ml
Na₂S·9H₂O	`0.5`	g
Distilled water	`970.0`	ml

Mix components except iron powder and filter-sterilize. Dispense the medium and iron powder (autoclaved) into sterile culture vessels (typically, 15 - 20 ml medium in 120 ml serum bottles) under a H₂-CO₂ (80:20, v/v) gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.01 volume of 5% Na₂S·9H₂O solution (neutralized with H₂SO₄, autoclaved and stored under a N₂ atmosphere). Pressurize the inoculated bottle to 200 kPa H₂-CO₂ (80:20, v/v).