Medium data

Search for medium no. = [216].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

216   

NH4Cl  0.25    g
K2HPO4  0.05    g
KH2PO4  0.09    g
NaCl  11.8     g
MgSO4·7H2O  1.75    g
MgCl2·6H2O  4.5     g
Na2SO4  0.81    g
CaCl2·2H2O  0.78    g
KCl  0.3     g
NaHCO3  1.0     g
Fe(NH4)2(SO4)2·6H2O  2.0     mg
(NH4)2Ni(SO4)2·6H2O  2.0     mg
Na2WO4·2H2O  2.0     mg
Trace minerals (see Medium No. 151)  10.0     ml
Marine trace elements (see below)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  1.0     mg
Na2S·9H2O  0.5     g
Distilled water  970.0     ml
Mix ingredients, except Na2S·9H2O, adjust pH to 6.0 with H2SO4. Sterilize by filtration while gassing with H2-CO2 (80:20) gas mixture. Autoclave Na2S·9H2O as a 5% solution under a N2 atmosphere. Prior to inoculation, anaerobically add the sterile Na2S·9H2O solution, and readjust pH to 6.5 with sterile H2SO4. Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20).
Marine trace elements solution:
NaBr  4.0     g
SrCl2·6H2O  1.8     g
H3BO3  1.3     g
KI  1.25    mg
Sodium silicate  100.0     mg
NaF  60.0     mg
KNO3  40.0     mg
Na2HPO4·12H2O  0.46    g
Distilled water  1.0     L