Medium data
Search for medium no. = [669].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
669 MAGNETOSPIRILLUM MEDIUM (B)
KH2PO4 | 0.28 | g |
NaNO3 | 0.12 | g |
Sodium thioglycolate | 0.05 | g |
Sodium tartrate | 0.37 | g |
Sodium succinate | 0.37 | g |
Sodium acetate | 0.05 | g |
Trace vitamins (see Medium No. 197) | 10.0 | ml |
Ferric quinate solution (see below) | 2.0 | ml |
Mineral salt solution (see Medium No. 304) | 1.0 | ml |
Distilled water | 1.0 | L |
Adjust pH to 6.75 with NaOH.
Ferric quinate solution:
FeCl3·6H2O | 0.45 | g |
Quinic acid | 0.19 | g |
Distilled water | 100.0 | ml |
Dissolve and autoclave at 121C for 15 min.
Comment: For strain JCM 21281, good growth can be obtained in semisolid medium (1.0 g/L agar).
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