Medium data
Search for medium no. = [304].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
304 PYGV MARINE MEDIUM (A)
| Bacto peptone (BD-Difco) | 0.25 | g |
| Yeast extract (BD-Difco) | 0.25 | g |
| Mineral salt solution (see below) | 20.0 | ml |
| 2.5% Glucose solution | 10.0 | ml |
| Vitamin solution (see below) | 10.0 | ml |
| Agar | 15.0 | g |
| Artificial seawater (see below) | 960.0 | ml |
Add components except the glucose solution and Vitamin solution, to artificial seawater and mix thoroughly. Gently heat and bring to a boil. Autoclave and then cool to 45 - 50C. Aseptically add the filter-sterilized glucose solution and Vitamin solution. Adjust pH to 7.5 with sterile KOH, if necessary.
Mineral salt solution:
| MgSO4·7H2O | 29.7 | g |
| Nitrilotriacetic acid | 10.0 | g |
| CaCl2·2H2O | 3.34 | g |
| FeSO4·7H2O | 99.0 | mg |
| Na2MoO4·2H2O | 13.0 | mg |
| Metals ''44'' (see Medium No. 149) | 50.0 | ml |
| Distilled water | 950.0 | ml |
Add nitrilotriacetic acid to 500 ml of distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add the remaining components and distilled water. Readjust pH to 7.2.
Vitamin solution:
| Biotin | 2.0 | mg |
| Folic acid | 2.0 | mg |
| Pyridoxine·HCl | 10.0 | mg |
| Riboflavin | 5.0 | mg |
| Thiamine·HCl | 5.0 | mg |
| Nicotinamide | 5.0 | mg |
| Calcium pantothenate | 5.0 | mg |
| Vitamin B12 | 0.1 | mg |
| p-Aminobenzoic acid | 5.0 | mg |
| Distilled water | 1.0 | L |
Artificial seawater:
| NaCl | 23.477 | g |
| Na2SO4 | 3.917 | g |
| MgCl2·6H2O | 4.981 | g |
| CaCl2·2H2O | 1.102 | g |
| NaHCO3 | 192.0 | mg |
| KCl | 664.0 | mg |
| KBr | 6.0 | mg |
| H3BO3 | 26.0 | mg |
| SrCl2·6H2O | 24.0 | mg |
| NaF | 3.0 | mg |
| Distilled water | 1.0 | L |
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