Medium data
Search for medium no. = [149].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
149 TETRAMETHYL AMMONIUM MEDIUM
| Tetramethyl ammonium | 1.0 | g |
| Thiamine·HCl | 0.5 | mg |
| Agar | 15.0 | g |
| Standard mineral base (see below) | 1.0 | L |
Standard mineral base:
| 1 M Na2HPO4-KH2PO4 buffer (pH 6.8) | 40.0 | ml |
| Hutner's vitamin free mineral base (see below) | 20.0 | ml |
| (NH4)2SO4 | 1.0 | g |
| Distilled water | 1.0 | L |
Hutner's vitamin free mineral base:
| Nitrilotriacetic acid | 10.0 | g |
| MgSO4·7H2O | 14.45 | g |
| CaCl2·2H2O | 3.335 | g |
| (NH4)6Mo7O24·4H2O | 9.25 | mg |
| FeSO4·7H2O | 99.0 | mg |
| Metals ''44'' (see below) | 50.0 | ml |
| Distilled water | 950.0 | ml |
After nitrilotriacetic acid is dissolved in distilled water and neutralized with ca. 7.3 g of KOH, add the remaining salts.
Metals ''44'':
| EDTA·2Na | 250.0 | mg |
| ZnSO4·7H2O | 1095.0 | mg |
| FeSO4·7H2O | 500.0 | mg |
| MnSO4·xH2O | 154.0 | mg |
| CuSO4·5H2O | 39.2 | mg |
| Co(NO3)2·6H2O | 24.8 | mg |
| Na2B4O7·10H2O | 17.7 | mg |
| Distilled water | 1.0 | L |
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved