Medium data

Search for medium no. = [149].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

149   TETRAMETHYL AMMONIUM MEDIUM

Tetramethyl ammonium  1.0     g
Thiamine·HCl  0.5     mg
Agar  15.0     g
Standard mineral base (see below)  1.0     L
Standard mineral base:
1 M Na2HPO4-KH2PO4 buffer (pH 6.8)  40.0     ml
Hutner's vitamin free mineral base (see below)  20.0     ml
(NH4)2SO4  1.0     g
Distilled water  1.0     L
Hutner's vitamin free mineral base:
Nitrilotriacetic acid  10.0     g
MgSO4·7H2O  14.45    g
CaCl2·2H2O  3.335   g
(NH4)6Mo7O24·4H2O  9.25    mg
FeSO4·7H2O  99.0     mg
Metals ''44'' (see below)  50.0     ml
Distilled water  950.0     ml
After nitrilotriacetic acid is dissolved in distilled water and neutralized with ca. 7.3 g of KOH, add the remaining salts.
Metals ''44'':
EDTA·2Na  250.0     mg
ZnSO4·7H2O  1095.0     mg
FeSO4·7H2O  500.0     mg
MnSO4·xH2O  154.0     mg
CuSO4·5H2O  39.2     mg
Co(NO3)2·6H2O  24.8     mg
Na2B4O7·10H2O  17.7     mg
Distilled water  1.0     L



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