Medium data

Search for medium no. = [661].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

661 MODIFIED AM5 MEDIUM

NaCl	`1.0`	g
KCl	`0.5`	g
MgCl₂·6H₂O	`0.4`	g
CaCl₂·2H₂O	`0.1`	g
NH₄Cl	`0.3`	g
KH₂PO₄	`0.2`	g
Na₂SO₄	`0.15`	g
Resazurin	`0.4`	mg
Distilled water	`910.0`	ml

Mix components thoroughly and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`40.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
Trace vitamins (see Medium No. 197)*	`10.0`	ml
Vitamin B₁₂ solution (see Medium No. 403)*	`1.0`	ml
Fatty acid mixture (see Medium No. 266)*	`1.0`	ml
10% Yeast extract solution	`10.0`	ml
10% Casamino acids solution	`10.0`	ml
1 M Glucose solution	`5.0`	ml
0.1 M Dithiothreitol solution*	`10.0`	ml