Medium data

Search for medium no. = [661].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

661   MODIFIED AM5 MEDIUM

NaCl  1.0     g
KCl  0.5     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.1     g
NH4Cl  0.3     g
KH2PO4  0.2     g
Na2SO4  0.15    g
Resazurin  0.4     mg
Distilled water  910.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  40.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
FeCl2 solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Trace vitamins (see Medium No. 197)*  10.0     ml
Vitamin B12 solution (see Medium No. 403)*  1.0     ml
Fatty acid mixture (see Medium No. 266)*  1.0     ml
10% Yeast extract solution  10.0     ml
10% Casamino acids solution  10.0     ml
1 M Glucose solution  5.0     ml
0.1 M Dithiothreitol solution*  10.0     ml