Medium data

Search for medium no. = [636].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
KH2PO4  0.54    g
Na2HPO4  1.34    g
Yeast extract (BD-Difco)  3.0     g
Tryptone (BD-Difco)  0.5     g
Resazurin  1.0     mg
Distilled water  860.0     ml
Solution B:
NH4Cl  0.19    g
NaCl  0.19    g
CaCl2·2H2O  0.006   g
MgCl2·6H2O  0.063   g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Distilled water  100.0     ml
Solution C:
50% Methanol  3.5     ml
Autoclave Solutions A and B separately and autoclave Solution C in a closed tube or vial. After cooling, combine the three solutions under a stream of N2-CO2 (80:20, v/v), then add the following solutions (filter-sterilized):
Trace vitamins (see Medium No. 197)  10.0     ml
8% NaHCO3 solution  30.0     ml
Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.025% L-cysteine·HCl·H2O and 0.025% Na2S·9H2O (autoclaved as 5% solutions under a N2 atmosphere).

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