Medium data

Search for medium no. = [631].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

631   MMB MEDIUM

Peptone  0.1     g
Yeast extract  0.1     g
Glucose  1.0     g
(NH4)2SO4  0.2     g
Hunter's basal salts (see below)  20.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Distilled water  1.0     L
Adjust pH to 7.0 - 7.5.
For preparation of solid medium, add 15.0 g/L agar.
Hunter's basal salts:
Nitrilotriacetic acid  10.0     g
MgSO4·7H2O  14.45    g
CaCl2·2H2O  4.42    g
(NH4)6Mo7O24·4H2O  9.25    mg
FeSO4·7H2O  99.0     mg
Nicotinic acid  50.0     mg
Thiamine·HCl  25.0     mg
Biotin  0.5     mg
Metals ''44'' (see Medium No. 149)  50.0     ml
Distilled water  950.0     ml
Dissolve and neutralized nitrilotriacetic acid in 500.0 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8 and bring volume to 1.0 L with distilled water.



Copyright © 2024 Microbe Division (JCM) - All Rights Reserved