Medium data
Search for medium no. = [607].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
607 HALOMONAS SUBGLACIESCOLA MEDIUM
NaCl | 100.0 | g |
MgCl2·6H2O | 5.0 | g |
MgSO4·7H2O | 9.5 | g |
KCl | 5.0 | g |
CaCl2·2H2O | 0.2 | g |
(NH4)2SO4 | 0.1 | g |
KNO3 | 0.1 | g |
Peptone | 5.0 | g |
Yeast extract | 1.0 | g |
Distilled water | 1.0 | L |
Adjust pH to 7.0. For preparation of solid medium, add 15.0 g/L agar. Autoclave. After cooling to 50C, add the following solutions to the medium (autoclaved or *filter-sterilized):
Metal solution (see below) | 20.0 | ml |
Phosphate solution (see below) | 20.0 | ml |
Trace vitamins* (see Medium No. 197) | 1.0 | ml |
Metal solution:
Nitrilotriacetic acid | 10.0 | g |
MgSO4·7H2O | 29.7 | g |
CaCl2·2H2O | 3.3 | g |
Na2MoO4·2H2O | 12.7 | mg |
FeSO4·7H2O | 99.0 | mg |
Metals ''44'' (see Medium No. 149) | 50.0 | ml |
Distilled water | 1.0 | L |
Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add the minerals. Adjust final pH to 7.0.
Phosphate solution:
K2HPO4 | 2.5 | g |
KH2PO4 | 2.5 | g |
Distilled water | 1.0 | L |
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