Medium data

Search for medium no. = [592].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Solution A:
NaCl  20.0     g
K2HPO4  0.09    g
KH2PO4  0.07    g
CaCl2·2H2O  0.8     g
NH4Cl  1.0     g
NaNO3  1.0     g
MgSO4·7H2O  1.0     g
MgCl2·6H2O  6.0     g
KCl  0.33    g
NiCl2·6H2O  1.0     mg
Na2SeO3·5H2O  1.0     mg
Fe2(SO4)3·xH2O  0.01    g
H2WO4  1.0     mg
Na2S2O3·5H2O  3.0     g
FeSO4·7H2O  0.01    g
Ferrous citrate  0.01    g
Trace mineral solution (see Medium No. 508)  5.0     ml
Distilled water  1.0     L
Mix ingredients and adjust pH to 6.8 with NaOH. Distribute the solution into culture vessels (leaving more than 80% volume of gas phase in the vessel) under a stream of a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Then, add the following solutions (filter-sterilized) to the medium (per liter):
Trace vitamins (see Medium No. 197)  1.0     ml
5% NaHCO3 solution  20.0     ml
Finally, replace the gas phase with a N2-CO2-O2 (79:20:1, by volume) gas mixture and pressurize to 200 kPa with the same gas mixture.