Medium data

Search for medium no. = [508].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  20.0     g
K2HPO4  0.09    g
KH2PO4  0.07    g
CaCl2·2H2O  0.8     g
NH4Cl  0.25    g
NaNO3  1.0     g
MgSO4·7H2O  4.0     g
MgCl2·6H2O  3.0     g
KCl  0.33    g
NiCl2·6H2O  1.0     mg
Na2SeO3·5H2O  1.0     mg
Fe2(SO4)3·xH2O  0.01    g
H2WO4  1.0     mg
Na2S2O3·5H2O  2.0     g
Sulfur (powder)  3.0     g
Trace mineral solution (see below)  10.0     ml
Trace vitamins (see Medium No. 197)  1.0     ml
NaHCO3  2.0     g
Distilled water  1.0     L
Mix ingredients except sulfur, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Replace the gas phase with N2-CO2-O2 (79:20:1, v/v) and pressurized inoculated bottles to 200 kPa with the same gas mixture.
Trace mineral solution:
Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4·2H2O.

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