Medium data
Search for medium no. = [508].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
508 MODIFIED MMJS MEDIUM
| NaCl | 20.0 | g |
| K2HPO4 | 0.09 | g |
| KH2PO4 | 0.07 | g |
| CaCl2·2H2O | 0.8 | g |
| NH4Cl | 0.25 | g |
| NaNO3 | 1.0 | g |
| MgSO4·7H2O | 4.0 | g |
| MgCl2·6H2O | 3.0 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 1.0 | mg |
| Na2SeO3·5H2O | 1.0 | mg |
| Fe2(SO4)3·xH2O | 0.01 | g |
| H2WO4 | 1.0 | mg |
| Na2S2O3·5H2O | 2.0 | g |
| Sulfur (powder) | 3.0 | g |
| Trace mineral solution (see below) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| NaHCO3 | 2.0 | g |
| Distilled water | 1.0 | L |
Mix ingredients except sulfur, Trace vitamins and NaHCO3, adjust pH to 6.8 and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) containing sulfur under a stream of N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Replace the gas phase with N2-CO2-O2 (79:20:1, v/v) and pressurized inoculated bottles to 200 kPa with the same gas mixture.
Trace mineral solution:
Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4·2H2O.
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