Medium data
Search for medium no. = [493].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
493 MMJ-HO MEDIUM
| Modified MJ synthetic seawater (see Medium No. 492) | 1.0 | L |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| NaNO3 | 1.0 | g |
| NaHCO3 | 1.0 | g |
| Sulfur (powder) | 3.0 | g |
Mix ingredients except NaHCO3 and sulfur, adjust pH to 7.0 with NaOH and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Pressurize the inoculated bottles to 200 kPa N2-CO2 (4:1, v/v).
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