Medium data

Search for medium no. = [430].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

430   DESULFOSPOROMUSA MEDIUM

Na2SO4  3.0     g
KH2PO4  0.2     g
NH4Cl  0.3     g
NaCl  1.0     g
MgCl2·6H2O  0.4     g
KCl  0.5     g
CaCl2·2H2O  0.15    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Resazurin  1.0     mg
Distilled water  946.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
1.0 M Sodium lactate solution*  20.0     ml
10% Yeast extract solution  10.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
8% NaHCO3 solution*  6.0     ml
5% Na2S·9H2O solution  8.0     ml
Adjust pH to 7.1 - 7.4, if necessary.