Medium data

Search for medium no. = [425].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

425 A1 MARINE MEDIUM

NaCl	`20.0`	g
KCl	`0.77`	g
NH₄Cl	`0.25`	g
KH₂PO₄	`0.1`	g
MgSO₄·7H₂O	`0.2`	g
Sodium acetate	`0.41`	g
Trace mineral solution (see Medium No. 411)	`10.0`	ml
Iron(III)citrate solution (see Medium No. 1014)	`50.0`	ml
Distilled water	`940.0`	ml

Mix components thoroughly and adjust pH to 6.8. Distribute the medium into culture vessels (e.g., 10ml; medium in Balch tubes) under a stream of N₂-CO₂ (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):

8%NaHCO₃ solution*	`25.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml
APM salts solution (see below)	`20.0`	ml

Readjust pH to 6.8, if necessary.
APM salts solution:

MgCl₂·6H₂O	`21.2`	g
CaCl₂·2H₂O	`3.04`	g
Distilled water	`1.0`	L