Medium data
Search for medium no. = [421].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
421 MMJHS MEDIUM
| MJ(-N) synthetic seawater (see Medium No. 268) | 1.0 | L |
| NaNO3 | 1.0 | g |
| Na2S2O3·5H2O | 1.0 | g |
| NaHCO3 | 1.0 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Sulfur (powder) | 3.0 | g |
Mix ingredients except sulfur, adjust pH to 6.5 - 7.0 with NaOH and filter-sterilize. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Comment: Adjust final pH to 7.0 (for strain JCM 12458) or 6.5 (for strain JCM 12459).
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