Medium data

Search for medium no. = [421].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

421   MMJHS MEDIUM

MJ(-N) synthetic seawater (see Medium No. 268)  1.0     L
NaNO3  1.0     g
Na2S2O3·5H2O  1.0     g
NaHCO3  1.0     g
Trace vitamins (see Medium No. 197)  10.0     ml
Sulfur (powder)  3.0     g
Mix ingredients except sulfur, adjust pH to 6.5 - 7.0 with NaOH and filter-sterilize. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v).
Comment: Adjust final pH to 7.0 (for strain JCM 12458) or 6.5 (for strain JCM 12459).