Medium data
Search for medium no. = [268].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
268 MJ/YPGS MEDIUM
| Yeast extract (BD-Difco) | 1.0 | g |
| Bacto peptone (BD-Difco) | 1.0 | g |
| Glucose | 0.2 | g |
| Sodium succinate | 0.2 | g |
| Na2SiO3·9H2O | 1.0 | g |
| Na2S2O3·5H2O | 2.48 | g |
| MJ(-N) synthetic seawater (see below) | 100.0 | ml |
| Distilled water | 900.0 | ml |
Adjust pH to 7.5.
MJ(-N) synthetic seawater:
| NaCl | 30.0 | g |
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 0.14 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 4.18 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 0.5 | mg |
| Na2SeO3·5H2O | 0.5 | mg |
| Fe(NH4)2(SO4)2·6H2O | 0.01 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Distilled water | 1.0 | L |
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