Medium data

Search for medium no. = [397].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

397 TD3 MEDIUM

Na₂SO₄	`4.0`	g
KH₂PO₄	`0.2`	g
NH₄Cl	`0.25`	g
NaCl	`20.0`	g
MgCl₂·6H₂O	`9.8`	g
KCl	`0.5`	g
CaCl₂·2H₂O	`0.1`	g
Na₂SeO₃	`6.0`	µg
Na₂WO₄·2H₂O	`8.0`	µg
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`20.0`	ml
Vitamin B₁₂	`0.1`	mg
Capronic acid	`0.5`	g
Fatty acid mixture (see Medium No. 266)	`10.0`	ml
NaHCO₃	`2.5`	g
Resazurin	`0.5`	mg
Na₂S·9H₂O	`0.4`	g
Distilled water	`1.0`	L

Mix ingredients, except capronic acid, Trace vitamins, vitamin B₁₂, NaHCO₃, and Na₂·9H₂O, and autoclave under a N₂-CO₂ (4:1, v/v) atmosphere. Add capronic acid to 10 ml distilled water and adjust pH to 7.5 with NaOH. Filter-sterilize the capronic acid solution, Fatty acid mixture and Trace vitamins with vitamin B₁₂, and store under a N₂ atmosphere. Filter-sterilize 5% NaHCO₃ solution and store under a N₂-CO₂ (4:1, v/v) atmosphere. Add the capronic acid solution, Fatty acid mixture, Trace vitamins and 5% NaHCO₃ solution to the medium. Prior to inoculation, add a 5% Na₂S·9H₂O solution (anaerobically autoclaved) to the medium. Adjust pH of the medium to 6.7 - 6.9, if necessary.