Medium data

Search for medium no. = [388].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

388 MV/LACTATE MEDIUM

NH₄Cl	`1.0`	g
Na₂SO₄	`2.0`	g
Na₂S₂O₃·5H₂O	`1.0`	g
MgSO₄·7H₂O	`1.0`	g
CaCl₂·2H₂O	`0.1`	g
KH₂PO₄	`0.5`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Sodium lactate	`1.0`	g
Yeast extract	`1.0`	g
NaHCO₃	`2.0`	g
Resazurin	`0.5`	mg
Na₂S·9H₂O	`75.0`	mg
Distilled water	`1.0`	L

Mix ingredients, except sodium lactate, NaHCO₃ and Na₂S·9H₂O, bring to a boil, and cool down to room temperature under a N₂ gas stream. Dispense the medium into culture vessels under a N₂ atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave sodium lactate, NaHCO₃ and Na₂S·9H₂O solutions (10, 8 and 0.75%, respectively) under a N₂ atmosphere, and add to the medium. Check final pH to be 7.0 - 7.2.