Medium data

Search for medium no. = [388].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

388   MV/LACTATE MEDIUM

NH4Cl  1.0     g
Na2SO4  2.0     g
Na2S2O3·5H2O  1.0     g
MgSO4·7H2O  1.0     g
CaCl2·2H2O  0.1     g
KH2PO4  0.5     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Sodium lactate  1.0     g
Yeast extract  1.0     g
NaHCO3  2.0     g
Resazurin  0.5     mg
Na2S·9H2O  75.0     mg
Distilled water  1.0     L
Mix ingredients, except sodium lactate, NaHCO3 and Na2S·9H2O, bring to a boil, and cool down to room temperature under a N2 gas stream. Dispense the medium into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. Separately autoclave sodium lactate, NaHCO3 and Na2S·9H2O solutions (10, 8 and 0.75%, respectively) under a N2 atmosphere, and add to the medium. Check final pH to be 7.0 - 7.2.