Medium data

Search for medium no. = [383].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

383   DESULFOBACTERIUM MEDIUM

Na2SO4  3.0     g
KH2PO4  0.2     g
NH4Cl  0.3     g
NaCl  30.0     g
MgCl2·6H2O  3.0     g
KCl  0.5     g
CaCl2·2H2O  0.15    g
Na2SeO3  3.0     µg
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Yeast extract (BD-Difco)  0.1     g
L-Sodium lactate  2.3     g
NaHCO3  2.5     g
Resazurin  1.0     mg
Na2S·9H2O  0.4     g
Distilled water  1.0     L
Mix ingredients, except L-sodium lactate, NaHCO3 and Na2S·9H2O, and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Separately, autoclave L-sodium lactate in 10 ml distilled water anaerobically and filter-sterilize 5% NaHCO3 gassed with N2-CO2 (4:1, v/v) for 20 min. Add the sterile L-sodium lactate and NaHCO3 solutions to the medium. Prior to inoculation, add an anaerobically autoclaved 5% Na2S·9H2O solution to the medium.