Medium data
Search for medium no. = [383].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
383 DESULFOBACTERIUM MEDIUM
| Na2SO4 | 3.0 | g |
| KH2PO4 | 0.2 | g |
| NH4Cl | 0.3 | g |
| NaCl | 30.0 | g |
| MgCl2·6H2O | 3.0 | g |
| KCl | 0.5 | g |
| CaCl2·2H2O | 0.15 | g |
| Na2SeO3 | 3.0 | µg |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Yeast extract (BD-Difco) | 0.1 | g |
| L-Sodium lactate | 2.3 | g |
| NaHCO3 | 2.5 | g |
| Resazurin | 1.0 | mg |
| Na2S·9H2O | 0.4 | g |
| Distilled water | 1.0 | L |
Mix ingredients, except L-sodium lactate, NaHCO3 and Na2S·9H2O, and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Separately, autoclave L-sodium lactate in 10 ml distilled water anaerobically and filter-sterilize 5% NaHCO3 gassed with N2-CO2 (4:1, v/v) for 20 min. Add the sterile L-sodium lactate and NaHCO3 solutions to the medium. Prior to inoculation, add an anaerobically autoclaved 5% Na2S·9H2O solution to the medium.
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