Medium data
Search for medium no. = [366].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
366 VULCANITHERMUS MEDIATLANTICUS MEDIUM
| NH4Cl | 0.33 | g |
| KCl | 0.33 | g |
| KH2PO4 | 0.33 | g |
| CaCl2·2H2O | 0.33 | g |
| MgCl2·6H2O | 0.33 | g |
| NaCl | 30.0 | g |
| Yeast extract (BD-Difco) | 0.3 | g |
| Sodium pyruvate | 2.0 | g |
| NaNO3 | 1.0 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Distilled water | 1.0 | L |
Mix ingredients except CaCl2·2H2O and MgCl2·6H2O, adjust pH to 6.5, and autoclave. Separately autoclave CaCl2·2H2O and MgCl2·6H2O, and add to the medium. Dispense the medium into culture vessels under a N2 atmosphere, and seal with butyl rubber stoppers.
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