Medium data
Search for medium no. = [356].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
356 MJ BASAL MEDIUM
| MJ(-N) synthetic seawater (see Medium No. 268) | 1.0 | L |
| NH4Cl | 0.25 | g |
| NaHCO3 | 1.5 | g |
| Na2S2O3·5H2O | 1.5 | g |
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
Mix ingredients, except NaHCO3, Na2S2O3·5H2O and Trace vitamins, and autoclave. Filter-sterilize 8% (w/v) NaHCO3, 10% (w/v) Na2S2O3·5H2O and Trace vitamins and add to the medium. Replace the gas phase with a N2-CO2-O2 (77:17:6, v/v) gas mixture, and pressurize to 200 kPa.
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