Medium data

Search for medium no. = [356].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

356   MJ BASAL MEDIUM

MJ(-N) synthetic seawater (see Medium No. 268)  1.0     L
NH4Cl  0.25    g
NaHCO3  1.5     g
Na2S2O3·5H2O  1.5     g
Trace vitamins (see Medium No. 197)  1.0     ml
Mix ingredients, except NaHCO3, Na2S2O3·5H2O and Trace vitamins, and autoclave. Filter-sterilize 8% (w/v) NaHCO3, 10% (w/v) Na2S2O3·5H2O and Trace vitamins and add to the medium. Replace the gas phase with a N2-CO2-O2 (77:17:6, v/v) gas mixture, and pressurize to 200 kPa.