Medium data
Search for medium no. = [344].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
344 HNW MEDIUM
| MJ(-N) synthetic seawater (see Medium No. 268) | 670.0 | ml |
| NH4Cl | 0.25 | g |
| NaNO3 | 1.0 | g |
| Na2WO4·2H2O | 0.1 | mg |
| Distilled water | 330.0 | ml |
Mix components thoroughly, adjust pH to 7.0 with NaOH and autoclave. After cooling, add the following solutions (filter-sterilized):
| 8% NaHCO3 solution | 12.5 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Comment: For JCM 34026, use 1.0 L of MJ(-N) synthetic seawater and omit distilled water.
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