Medium data

Search for medium no. = [320].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

320   THERMOTOGA YE MEDIUM

Yeast extract (BD-Difco)  2.0     g
NaCl  20.0     g
MgCl2·6H2O  3.0     g
MgSO4·7H2O  6.0     g
(NH4)2SO4  1.0     g
CaCl2·2H2O  0.3     g
KH2PO4  0.2     g
KCl  0.5     g
NaBr  0.05    g
H3BO3  0.025   g
SrCl2·6H2O  0.02    g
Ferric citrate  0.01    g
Trace minerals (see Medium No. 151)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients, except yeast extract and Na2S·9H2O, adjust pH to 6.5 with H2SO4, and filter-sterilize under a N2 atmosphere. Separately autoclave 10% yeast extract solution and neutralized 5% Na2S·9H2O solution under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution to the medium. Prior to inoculation, add the Na2S·9H2O solution to the medium, and readjust pH to 6.5 - 7.0, if necessary. Cultivate under a N2 atmosphere.