Medium data
Search for medium no. = [320].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
320 THERMOTOGA YE MEDIUM
| Yeast extract (BD-Difco) | 2.0 | g |
| NaCl | 20.0 | g |
| MgCl2·6H2O | 3.0 | g |
| MgSO4·7H2O | 6.0 | g |
| (NH4)2SO4 | 1.0 | g |
| CaCl2·2H2O | 0.3 | g |
| KH2PO4 | 0.2 | g |
| KCl | 0.5 | g |
| NaBr | 0.05 | g |
| H3BO3 | 0.025 | g |
| SrCl2·6H2O | 0.02 | g |
| Ferric citrate | 0.01 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients, except yeast extract and Na2S·9H2O, adjust pH to 6.5 with H2SO4, and filter-sterilize under a N2 atmosphere. Separately autoclave 10% yeast extract solution and neutralized 5% Na2S·9H2O solution under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution to the medium. Prior to inoculation, add the Na2S·9H2O solution to the medium, and readjust pH to 6.5 - 7.0, if necessary. Cultivate under a N2 atmosphere.
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