Medium data

Search for medium no. = [280].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

280   THERMOCOCCUS MEDIUM

KCl  0.33    g
MgCl2·6H2O  2.8     g
MgSO4·7H2O  3.4     g
NH4Cl  0.25    g
NaCl  25.0     g
K2HPO4  0.3     g
Yeast extract (BD-Difco)  3.0     g
Tryptone (BD-Difco)  3.0     g
FeSO4·7H2O  0.025   g
NaBr  10.0     mg
Trace minerals (see Medium No. 151)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Sulfur (powder)  10.0     g
Na2S·9H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients except sulfur and Na2S·9H2O, adjust pH to 7.0-7.2 and autoclave under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S·9H2O as a 5% solution and autoclave under a N2 gas atmosphere. Distribute the medium and sulfur into culture vessels under a N2 stream, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S·9H2O solution, and readjust pH to 7.0-7.2, if necessary.
Comment:
Most of Thermococcus strains will grow in this medium supplemented with 5.0 g/L maltodextrin and omitted sulfur.