Medium data
Search for medium no. = [280].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
280 THERMOCOCCUS MEDIUM
| KCl | 0.33 | g |
| MgCl2·6H2O | 2.8 | g |
| MgSO4·7H2O | 3.4 | g |
| NH4Cl | 0.25 | g |
| NaCl | 25.0 | g |
| K2HPO4 | 0.3 | g |
| Yeast extract (BD-Difco) | 3.0 | g |
| Tryptone (BD-Difco) | 3.0 | g |
| FeSO4·7H2O | 0.025 | g |
| NaBr | 10.0 | mg |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Sulfur (powder) | 10.0 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix ingredients except sulfur and Na2S·9H2O, adjust pH to 7.0-7.2 and autoclave under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S·9H2O as a 5% solution and autoclave under a N2 gas atmosphere. Distribute the medium and sulfur into culture vessels under a N2 stream, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S·9H2O solution, and readjust pH to 7.0-7.2, if necessary.
Comment:
Most of Thermococcus strains will grow in this medium supplemented with 5.0 g/L maltodextrin and omitted sulfur.
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