Medium data
Search for medium no. = [255].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
255 AQUIFEX MEDIUM
| NaCl | 30.0 | g |
| MgSO4·7H2O | 7.0 | g |
| MgCl2·6H2O | 5.5 | g |
| KCl | 0.65 | g |
| NaBr | 0.1 | g |
| NaHCO3 | 2.0 | g |
| NH4Cl | 0.15 | g |
| K2HPO4 | 0.15 | g |
| CaCl2·2H2O | 0.5 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| (NH4)2Ni(SO4)2·6H2O | 20.0 | mg |
| Na2WO4·2H2O | 0.1 | mg |
| Na2SeO4 | 0.1 | mg |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Distilled water | 1.0 | L |
Adjust pH to 6.0. Sterilize by filtration. Aseptically dispense the medium into culture vessels (e.g., 20 ml medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Replace the gas phase with a H2-CO2-O2 (79:20:1, v/v) gas mixture and pressurize to 200 kPa with the same gas mixture. Readjust pH to 5.5-6.0, if necessary.
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