Medium data

Search for medium no. = [242].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

242

KH₂PO₄	`0.3`	g
K₂HPO₄	`0.3`	g
(NH₄)₂SO₄	`0.3`	g
NaCl	`0.6`	g
MgSO₄·7H₂O	`0.13`	g
FeSO₄·7H₂O	`2.0`	mg
CaCl₂·2H₂O	`8.0`	mg
Yeast extract (BD-Difco)	`2.0`	g
Trypticase peptone (BD-BBL)	`2.0`	g
Sodium acetate	`2.5`	g
Sodium formate	`2.5`	g
Trace minerals (see Medium No. 151)	`10.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
NaHCO₃	`5.0`	g
Na₂S·9H₂O	`0.5`	g
L-Cysteine·HCl·H₂O	`0.5`	g
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Mix ingredients, except NaHCO₃, L-Cysteine·HCl·H₂O and Na₂S·9H₂O, and bring to a boil for 5-10 sec. Cool down under a stream of H₂-CO₂ (80:20), and add NaHCO₃ to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H₂-CO₂ (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine·HCl and Na₂S·9H₂O as 5% solutions, respectively, under a N₂ atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine·HCl and Na₂S·9H₂O solutions. Pressurize the inoculated vessels to 200 kPa H₂-CO₂ (80:20).