Medium data

Search for medium no. = [242].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

242   

KH2PO4  0.3     g
K2HPO4  0.3     g
(NH4)2SO4  0.3     g
NaCl  0.6     g
MgSO4·7H2O  0.13    g
FeSO4·7H2O  2.0     mg
CaCl2·2H2O  8.0     mg
Yeast extract (BD-Difco)  2.0     g
Trypticase peptone (BD-BBL)  2.0     g
Sodium acetate  2.5     g
Sodium formate  2.5     g
Trace minerals (see Medium No. 151)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
NaHCO3  5.0     g
Na2S·9H2O  0.5     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Distilled water  1.0     L
Mix ingredients, except NaHCO3, L-Cysteine·HCl·H2O and Na2S·9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20), and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine·HCl and Na2S·9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine·HCl and Na2S·9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).