Medium data
Search for medium no. = [232].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
232 METHANOCALDOCOCCUS JANNASCHII MEDIUM
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 0.14 | g |
| NH4Cl | 0.25 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 2.7 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 0.75 | mg |
| Na2SeO3·5H2O | 0.5 | mg |
| NaCl | 30.0 | g |
| Fe(NH4)2(SO4)2·6H2O | 0.01 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| NaHCO3 | 1.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Na2S·9H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 980.0 | ml |
Mix ingredients, except NaHCO3, cysteine·HCl and Na2S·9H2O, and adjust pH to 6.0. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20) and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine·HCl and Na2S·9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine·HCl and Na2S·9H2O solutions. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20).
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