Medium data
Search for medium no. = [1444].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1444 DESULFOSPOROSINUS SB140 MEDIUM
| (NH4)2SO4 | 0.45 | g |
| KH2PO4 | 0.05 | g |
| MgSO4-7H2O | 0.5 | g |
| KCl | 0.05 | g |
| Yeast extract | 0.5 | g |
| Ca(NO3)2•4H2O | 0.014 | g |
| Na2SO4 | 3.0 | g |
| Trace element solution (see Medium No. 439) | 1.0 | ml |
| L-Cysteine•HCl•H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Distilled water | 925.0 | ml |
Mix components thoroughly, adjust pH to 7.0, and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized).
| 5% Na2HPO4 solution | 40.0 | ml |
| 5% KH2PO4 solution | 20.0 | ml |
| 1M Glycerin solution | 5.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
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