Medium data
Search for medium no. = [1420].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1420 PFENNIG IGNISPHAERA MEDIUM
| NH4Cl | 0.33 | g |
| KH2PO4 | 0.33 | g |
| KCl | 0.33 | g |
| CaCl2·2H2O | 0.33 | g |
| MgCl2·6H2O | 0.33 | g |
| Yeast extract (Oxoid) | 0.2 | g |
| Beef extract | 0.5 | g |
| Trace element solution (see Medium No. 899) | 1.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly, adjust pH to 7.0, and autoclave under a N2 atmosphere. Aseptically and anaerobically add per liter the following solution (autoclaved and stored under a N2 gas atmosphere):
| 5% Na2S•9H2O solution | 6.0 | ml |
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