Medium data

Search for medium no. = [899].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

899   THERMOBACTER ROSEUM MEDIUM

KH2PO4  0.2     g
NH4Cl  0.25    g
NaCl  1.0     g
MgCl2·6H2O  0.4     g
KCl  0.5     g
CaCl2·2H2O  0.1     g
NaHCO3  0.1     g
Resazurin  1.0     mg
Yeast extract (BD-Difco)  2.0     g
Distilled water  1.0     L
Mix components thoroughly and autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
Vitamin solution* (see below)  1.0     ml
Trace element solution* (see below)  1.0     ml
5% Na2S·9H2O  6.0     ml
Check pH to be 7.5 - 7.6.
Vitamin solution:
p-Aminobenzoic acid  25.0     mg
Biotin  100.0     mg
Nicotinic acid  25.0     mg
DL-Calcium pantothenate  25.0     mg
Pyridoxine·HCl  25.0     mg
Folic acid  10.0     mg
Riboflavin  25.0     mg
Vitamin B12  0.5     mg
Lipoic acid  25.0     mg
Distilled water  1.0     L
Trace element solution:
(NH4)2Fe(SO4)2·6H2O      784.0      mg
25% HCl       5.0        ml
CoCl2·6H2O    238.0      mg
(NH4)2Ni(SO4)2•6H2O       395.0      mg
Na2MoO4·2H2O        36.0       mg
Na2WO4·2H2O   33.0       mg
ZnSO4·7H2O    144.0      mg
CuCl2·2H2O    2.0        mg
Na2SeO4       94.0       mg
H3BO3 6.0        mg
MnCl2·4H2O    99.0       mg
Distilled water     995.0      ml
Dissolve (NH4)2Fe(SO4)2·6H2O in 25% HCl first, then dilute in distilled water. Add the remaining salts in the sequence indicated



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