Medium data

Search for medium no. = [899].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

899   THERMOBACTER ROSEUM MEDIUM

KH2PO4  0.2     g
NH4Cl  0.25    g
NaCl  1.0     g
MgCl2·6H2O  0.4     g
KCl  0.5     g
CaCl2·2H2O  0.1     g
NaHCO3  0.1     g
Resazurin  1.0     mg
Yeast extract (BD-Difco)  2.0     g
Distilled water  1.0     L
Mix components thoroughly and autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
Vitamin solution* (see below)  1.0     ml
Trace element solution* (see below)  1.0     ml
5% Na2S·9H2O  6.0     ml
Check pH to be 7.5 - 7.6.
Vitamin solution:
p-Aminobenzoic acid  25.0     mg
Biotin  100.0     mg
Nicotinic acid  25.0     mg
DL-Calcium pantothenate  25.0     mg
Pyridoxine·HCl  25.0     mg
Folic acid  10.0     mg
Riboflavin  25.0     mg
Vitamin B12  0.5     mg
Lipoic acid  25.0     mg
Distilled water  1.0     L
Trace element solution:
Fe(NH4)2(SO4)2·6H2O  784.0     mg
CoCl2·6H2O  238.0     mg
(NH4)2Ni(SO4)2·6H2O  395.0     mg
Na2MoO4·2H2O  24.0     mg
Na2WO4·2H2O  33.0     mg
ZnSO4·7H2O  144.0     mg
CuCl2·2H2O  2.0     mg
Na2SeO4  94.0     mg
H3BO3  6.0     mg
MnCl2·4H2O  99.0     mg
Distilled water  1.0     L