Medium data

Search for medium no. = [1415].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1415 METHANOSARCINA Z-7115 MEDIUM

NH₄Cl	`0.33`	g
CaCl₂·2H₂O	`0.16`	g
KH₂PO₄	`0.33`	g
MgCl₂·6H₂O	`0.33`	g
Sodium acetate	`0.2`	g
Trace element solution (see Medium No. 899)	`1.0`	ml
Resazurin	`0.5`	mg
Distilled water	`960.0`	ml

Mix components and adjust pH to 7.0. Autoclave the medium under a N₂-CO₂ (80:20 v/v) gas mixture. After cooling, aseptically and anaerobically, add the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ soluiton*	`25.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml
50% (v/v) Methanol	`4.0`	ml

Dispense the medium into culture vessels (e.g., 20 ml of the medium in 60 ml serum bottles) under the same gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solutions (autoclaved and stored under a N₂ atmosphere) :

5% L-Cysteine·HCl·H₂O solution	`5.0`	ml
5% Na₂S·9H₂O solution	`5.0`	ml