Medium data

Search for medium no. = [1405].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1405 THIOHALORHABDUS METHYLOTROPHUS MEDIUM

NaCl	`180.0`	g
KCl	`5.0`	g
K₂HPO₄	`2.5`	g
NH₄Cl	`0.2`	g

Add components to distilled water and bring volume to 1.0 L. Adjust pH to 6.8 by 10% K₂HPO₄ or KH₂PO₄ solution, and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):

1 M MgSO₄ solution	`1.0`	ml
Trace element solution (see Medium No. 1079)	`1.0`	ml
0.002% CuCl₂·2H₂O solution	`1.0`	ml
Trace vitamins* (see Medium No. 197)	`5.0`	ml
3% Trimethylamine solution*	`20.0`	ml
2 M Sodium thiosulfate solution*	`1.0`	ml

Place the medium in culture vessels leaving >80% head space (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Cultivated at the designated temperature with shaking.