Medium data

Search for medium no. = [1367].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1367   MARINE DESULFOVIBRIO COMPLEX MEDIUM

Sea salts (Sigma)  35.0     g
Wolfe's mineral elixir (see Medium No. 470)  1.0     ml
Yeast extract (BD-Difco)  1.0     g
Resazurin  0.5     mg
Distilled water  945.0     ml
Mix components thoroughly and autoclave under a N2 atmosphere. Aseptically and anaerobically add the following solutions from sterile anaerobic stock solutions (autoclaved or *filter-sterilized):
1 M Sodium lactate solution  20.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
8% NaHCO3 solution*  25.0     ml
Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a stream of N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Finally add per liter the following solution (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  6.0     ml



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