Medium data
Search for medium no. = [1351].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1351 PELOSINUS BKL1 MEDIUM
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.2 | g |
| NaH2PO4·2H2O | 0.91 | g |
| CaCl2·2H2O | 0.1 | g |
| NH4Cl | 1.0 | g |
| Yeast extract (BD-Difco) | 0.2 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Distilled water | 925.0 | ml |
Mix components thoroughly and adjust pH to 7.2. Sparge the medium with a N2-CO2 (4:1, v/v) gas mixture and autoclave under the same gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
| 8.0% NaHCO3 solution* | 30.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| 1.0 M Sodium lactate solution | 30.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 5.0 | ml |
Readjust pH to 7.0-7.2, if necessary.
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