Medium data

Search for medium no. = [1347].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1347   FRESHWATER THIOSULFATE-OXIDIZING BACTERIA MEDIUM

Modified Wolfe's solution (see Medium No. 628)  1.0     L
Trace minerals (see Medium No. 151)  10.0     ml
Mix components and autoclave. Aseptically add per liter the following solutions from sterile stocks (autoclaved or *filter-sterilized):
1M MES solution  10.0     ml
1M NaHCO3 solution*  5.0     ml
Trace vitamins solution* (see Medium No. 197)  10.0     ml
1 M Na2S2O3·5H2O solution*  10.0     ml
Check final pH to be 6.0-6.5. Dispense into culture vessels, replace the gas phase with a N2-CO2-O2 (79:20:1, v/v/v) gas mixture, and seal with butyl rubber stoppers.
Modified Wolfe's Mineral solution:
NH4Cl  1.0     g
MgSO4·7H2O  0.2     g
CaCl2·2H2O  0.1     g
KH2PO4  0.05    g
Distilled water  1.0     L
Trace minerals:
Nitrilotriacetic acid  1.5     g
MgSO4·7H2O  3.0     g
MnSO4·xH2Ov  0.5     g
NaCl  1.0     g
FeSO4·7H2O  0.1     g
CoSO4·7H2O  0.1     g
CaCl2·2H2O  0.1     g
ZnSO4·7H2O  0.1     g
CuSO4·5H2O  0.01    g
AlK(SO4)2  0.01    g
H3BO3  0.01    g
Na2MoO4·2H2O  0.01    g
Distilled water  1.0     L
Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
Trace vitamins:
Biotin  2.0     mg
Folic acid  2.0     mg
Pyridoxine·HCl  10.0     mg
Thiamine·HCl  5.0      mg
Riboflavin  5.0     mg
Nicotinic acid  5.0     mg
DL-Calcium pantothenate  5.0     mg
Vitamin B12  0.1     mg
p-Aminobenzoic acid  5.0     mg
Lipoic acid  5.0     mg
Distilled water  1.0     L