Medium data
Search for medium no. = [1345].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1345 SULFURIFLEXUS MEDIUM
| NaCl | 20.0 | g |
| MgCl2·6H2O | 3.0 | g |
| MgSO4·7H2O | 0.3 | g |
| CaCl2·2H2O | 0.14 | g |
| NH4Cl | 0.1 | g |
| KH2PO4 | 0.1 | g |
| KCl | 0.1 | g |
| Na2HPO4 | 0.6 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Selenite tungstate solution (see below) | 1.0 | ml |
| Na2S2O3·5H2O | 5.0 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| NaHCO3 | 0.4 | g |
| Distilled water | 1.0 | L |
Dissolve ingredients (except thiosulfate, bicarbonate and vitamins), adjust pH to 6.0 and dispense medium under air atmosphere into serum vials up to a volume of 20%, close with rubber septa and autoclave. Add thiosulfate, vitamins and bicarbonate to the autoclaved medium from sterile stock solutions sterilized by filtration. Adjust pH of the complete medium to 7.2.
Selenite tungstate solution:
| Na2SeO3·5H2O | 3.0 | mg |
| Na2WO4·2H2O | 4.0 | mg |
| Distilled water | 1.0 | L |
Trace vitamins:
| Biotin | 2.0 | mg |
| Folic acid | 2.0 | mg |
| Pyridoxine·HCl | 10.0 | mg |
| Thiamine·HCl | 5.0 | mg |
| Riboflavin | 5.0 | mg |
| Nicotinic acid | 5.0 | mg |
| DL-Calcium pantothenate | 5.0 | mg |
| Vitamin B12 | 0.1 | mg |
| p-Aminobenzoic acid | 5.0 | mg |
| Lipoic acid | 5.0 | mg |
| Distilled water | 1.0 | L |
FeCl2 solution:
| 25% HCl (7.7 M) | 10.0 | ml |
| FeCl2·4H2O | 1.5 | g |
| Distilled water | 990.0 | ml |
Trace element solution:
| ZnCl2 | 70.0 | mg |
| MnCl2·4H2O | 100.0 | mg |
| H3BO3 | 6.0 | mg |
| CoCl2·6H2O | 190.0 | mg |
| CuCl2·2H2O | 2.0 | mg |
| NiCl2·6H2O | 24.0 | mg |
| Na2MoO4·2H2O | 36.0 | mg |
| Distilled water | 1.0 | L |
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